Yinlingtong capsules reduced the accumulation of fat in HHL-5 hepatocytes

VernacularTitle:银灵通胶囊减少人肝细胞系-5细胞的脂肪积聚作用及机制研究
Author: Guining LIANG ; Zhenjun XU ; Leihua WENG ; Yayi HOU
Publication Type:Journal Article
Keywords: Triglyceride; Fatty acid oxidation level; CPT-1β; ACC-β
From: International Journal of Traditional Chinese Medicine 2013;(3):225-227
CountryChina
Language:Chinese
Abstract: Objective To study the effects of the Yinlingtong capsules on intracellular triglyceride,fatty acid oxidation levels and CPT-1/ACC-β in HHL-5 cells.Methods After treated HHL-5 cells with different doses Yinlingtong capsules,intracellular triglyceride content and the fatty acid oxidation level were detected by the kits.The CPT-1β and ACC-β mRNA levels were detected by Q-PCR.Results Yinlingtong capsules could reduce triglyceride content (compared with the control group,triglyceride content of0.5,1 and 2 mg/ml dose group were reduced by 9.5％,14.3％ and 19.4％,respectively),enhance the level of fatty acid oxidation by a dose-response relationship(compared with the control group,the level of fatty acid oxidation of 0.5,1and 2 mg/ml dose group were increased 4.3％,6.9％ and 11.2％,respectively),but could not affect the CPT-1βand ACC-β mRNA levels.Conclusion Yinlingtong capsules reduced the accumulation of fat in HHL-5 hepatocytes,and the specific impact mechanism needs to be further explored.