Photodynamic activity of novel targeting photosensitizer Ⅰ for Hep-2 cells
10.3760/cma.j.issn.1006-9801.2013.01.001
- VernacularTitle:新型靶向光敏剂Ⅰ对Hep-2细胞的光动力抑瘤活性
- Author:
Donghong LI
;
Pengxi LI
;
Zonglin JIANG
;
Linfeng GUO
- Publication Type:Journal Article
- Keywords:
Photosensitizer Ⅰ;
Hep-2 cells;
Tumor targeting;
Photodynamic activity
- From:
Cancer Research and Clinic
2013;(1):1-4
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the targeting and photodynamic activity of photosensitizer Ⅰ (PSⅠ)for laryngocarcinoma Hep-2 cells.Methods The photostability of PSⅠ was measured by bleaching assay.The cellular uptake of PSⅠ by Hep-2 cells were evaluated by fluorescence spectroscopy,the effects of PSⅠconcentration,irradiation dose and etc on the viability of Hep-2 cells were investigated by MTT assay.Results After irradiation of 50 min,the OD value of PS Ⅰ decreased 11％,which indicated that the stability of PSⅠ can meet the requirement of PDT.The cellular uptake of PS Ⅰ by Hep-2 cells increased with the concentration of PSⅠ and can be obviously inhibited by the presence of excessive free folic acid (P ＜ 0.01).The results of MTT assays demonstrated that the viability of Hep-2 showed negative correlation with the PSⅠ concentration and irradiation dose.The viability of Hep-2 was only 34 ％ after PDT with 14 μ mol/L of the PS Ⅰ and 18 J/cm2 of irradiation.However,the viability of Hep-2 was still 100 ％ without irradiation,even though the concentration of PS Ⅰ was up to 1 10 μmol/L.The PDT carried out after 24 h of PSⅠ administration can efficiently inhibited the growth of Hep-2 with the survival rate of 32 ％.Conclusion The PS Ⅰ posses satisfactory photostability and lower dark cytotoxicity,and displays significant targeting and photocytotoxicity for Hep-2 cells.
