Research on induced apoptosis of recombinant human adenovirus-p53 injection in patients with oral leukoplakia
10.3760/cma.j.issn.1006-9801.2013.02.011
- VernacularTitle:重组人p53腺病毒注射液治疗口腔白斑诱导病变细胞凋亡的研究
- Author:
Songtao ZHANG
;
Yuanyuan ZHANG
;
Zhuang ZHANG
;
Xiaoyu LI
;
Longjiang LI
- Publication Type:Journal Article
- Keywords:
Adenoviruses,human;
Gene therapy;
Leukoplakia,oral;
Apoptosis;
Terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling assay
- From:
Cancer Research and Clinic
2013;(2):107-109
- CountryChina
- Language:Chinese
-
Abstract:
Objective To probe the biological effect of multiple intraepithelial injections of recombinant adenovirus-p53(rAd-p53)on inducing the apoptosis in patients with dysplastic oral leukoplakia.Methods 18 patients clinically and histopathologically diagnosed as dysplastic oral leukoplakia were recruited for this study.Intraepithelial injections of rAd-p53 were administered.Immunohistochemistry was used to examine the protein expression of p53 and bcl-2.TUNEL was performed to detect apoptotic cells.Results In the post-treatment patients,p53 protein expression were significantly enhanced(100 ％,18/18),yet bcl-2 protein presented low expression(17 ％,3/18).Statistical analysis revealed the expression of p53 protein had a negative correlation with that of bcl-2 protein(r =-0.837,P ＜ 0.01).15 post-treatment samples (83 ％)were detected obvious apoptotic cells,especially in the samples that were strong p53-positive(r =0.684,P ＜ 0.01).Conclusion Intraepithelial injections of rAd-p53 can induce apoptosis for patients with dysplastic oral leukoplakia.It may be a promising treatment for oral leukoplakia.
