Effect of oridonin on the gene expression of human esophageal carcinoma cell SHEEC and the target gene screening
10.3760/cma.j.issn.1006-9801.2013.02.001
- VernacularTitle:冬凌草甲素对人类食管癌SHEEC细胞基因表达的影响及其靶基因筛选
- Author:
Shaobin WANG
;
Wanzhong HUANG
;
Yu ZHONG
;
Xiaojie LI
;
Junhui CHEN
- Publication Type:Journal Article
- Keywords:
Oridonin;
Human esophageal carcinoma cell SHEEC;
Gene chip;
Fluorecent quantitative polymerase chain reaction
- From:
Cancer Research and Clinic
2013;(2):73-76
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the effect of oridonin(ORI)on the gene expression of human esophageal carcinoma cell SHEEC and to screen the tumor cell apoptosis target genes.Methods The gene expression of human esophageal carcinoma cell SHEEC without and with ORI induction for 1 hours and 8 hours were detected with microarray technique,respectively.The differentially expressed genes were identified and verified with fluorecent quantitative PCR.Results A total of 1011 genes showed up or down regulation more than twice after ORI induction(including 280 genes after 1 hour and 731 genes after 8 hours induction respectively).In these genes,17 genes with the top extent of up or down regulation were identified,which were involved in the cell signal transduction,transcription regulation,and cell apoptosis.These 17 differentially expressed genes were verified with real-time PCR,and 12 genes were statistically significant.Conclusion In the 12 differentially expressed genes with statistically significance,there may have tumor cell apoptosis target genes induced by ORI through mitochondrion route.
