Observation on effect of Livin on VSV-induced apoptosis of A549 cells
10.3760/cma.j.issn.1006-9801.2013.01.002
- VernacularTitle:Livin影响水疱口炎病毒诱导肺癌A549细胞凋亡效应的实验观察
- Author:
Xiaolong ZHANG
;
Tao ZHANG
;
Huanyi LIU
;
Zhenguo ZHAO
- Publication Type:Journal Article
- Keywords:
Vesicular stomatitis virus;
A549 cells;
RNA interference;
Livin
- From:
Cancer Research and Clinic
2013;(1):5-7
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of Livin expression on VSV-induced apoptsis of A549 cells.Methods The expression of Livin of A549 cells was inhibited by RNA interference.VSV-induced apoptosis of A549 cells was observed by Tunel assay.Protein Level of livin was detected by Western blot.Caspase-3 activity was detected by the fluorescence-based quantitative method.Results Livin downregulation VSV-induced apoptosis of A549 cells.Inhibited the expression of Livin of A549 cells had increased Caspase-3 activity.Conclusion The effect of Livin on VSV-induced apoptotic of A549 cells could be increased by RNA interference.
