Interaction effects of DNA methylation and histone modification key enzymes in gastric cancer
10.3760/cma.j.issn.0254-1432.2013.02.008
- VernacularTitle:DNA甲基化和组蛋白修饰的关键酶在胃癌中存在相互作用
- Author:
Fuli GAO
;
Ying Lü
;
Yinxin ZHU
;
Jun CAO
;
Xiaoping ZOU
- Publication Type:Journal Article
- Keywords:
DNA (cytosine 5)-methyhransferase;
Methyltransferases;
Histones;
Histone deacetylases;
Stomach neoplasms
- From:
Chinese Journal of Digestion
2013;(2):106-110
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression and interactions of DNA methyltransferase 1 (DNMT1),enhancer of zeste homolog 2 (EZH2) and histone deacetylase 1 (HDAC1) in gastric cancer cell lines and tissue specimens.Methods The expression of DNMT1,EZH2 and HDAC1 was detected at mRNA and protein level in gastric cancer lines MKN28,SGC7901,BGC823,AGS,normal gastric epithelium cell line GES-1 and 10 pairs of fresh gastric cancer tissues and corresponding normal gastric tissues by real-time polymerase chain reaction and Western blot.Whether DNMT1,EZH2 and HDAC1 forming complex or not was detected by co-immunoprecipitation (Co-IP) in well-differentiated gastric cancer cell line MKN28,medium-differentiated gastric cancer cell line SGC7901,lowdifferentiated gastric cancer cell line BCG823,normal gastric epithelium cell line GES-1,mediumdifferentiated,medium to low-differentiated,low-differentiated gastric cancer tissues and corresponding normal gastric tissues.Results Compared with that of normal gastric epithelium cell and gastric tissue,the expression of DNMT1,EZH2 and HDAC1 in gastric cancer cell lines and gastric tissue was higher.The results of Co-IP indicated that DNMT1,EZH2 and HDAC1 formed complex in the high,medium,and poor differentiated gastric cancer cells and the medium,mediumlow,poor differentiated gastric cancer tissues,but not in normal gastric epithelium cell and tissue.Conclusion DNMT1,EZH2 and HDAC1 highly expressed in gastric cancer and there was interaction effects among them,which might be an important mechanism in the correlation between DNA methylation and histone modifications in gastric cancer.
