Expression and distribution of aquaporin 3 during early embryonic development of mouse
10.3760/cma.j.issn.1007-9408.2013.02.006
- VernacularTitle:水通道蛋白3在小鼠早期胚胎发育过程中的表达
- Author:
Yingqi NONG
;
Fenghua LIU
;
Ye CHEN
;
Yitong LIU
;
Jianxing RUAN
- Publication Type:Journal Article
- Keywords:
Aquaporin 3;
Embryonic development;
Microscopy,fluorescence;
Mice
- From:
Chinese Journal of Perinatal Medicine
2013;(2):86-89
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression and distribution of aquaporin 3 (AQP3) in mouse early embryos at different stages.Methods Controlled ovarian hyperstimulation model of Kunming mouse was used to collect four-cell embryos,eight-cell embryos,morula stage,and early blastocysts.Immunofluorescence microscopy and laser confocal microscopy were used to detect expression and distribution of AQP3 channels in these stages.Results Fluorescence signal of AQP3 was found in four embryonic stages of mice.Distribution within embryo was different at different embryonic stages.AQP3 was mainly expressed on the karyotheca of blastomeres at four-cell and eight-cell stage.In morula stage,AQP3 was mainly expressed on cell membrane of each blastomere.In early blastocysts,AQP3 was predominantly expressed on the cell membrane and cytoplasm of trophoblastic cell.Conclusions AQP3 trans-membrane channel might have potential regulation function on mouse embryonic development.
