Expression and its significance of stem cells marker leucine-rich repeat containing G protein coupled receptor 5 gene in human colorectal cancer
10.3760/cma.j.issn.0254-1432.2013.03.011
- VernacularTitle:人结直肠癌干细胞标志物富含亮氨酸重复单位的G蛋白耦联受体5基因的表达及意义
- Publication Type:Journal Article
- Keywords:
Colorectal neoplasms;
Stem cells;
lgr5;
Gene expression
- From:
Chinese Journal of Digestion
2013;(3):187-190
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of stem cell marker leucine-rich repeat containing G protein coupled receptor 5 (lgr5) gene in human colorectal cancer tissues and peripheral blood and its correlation with clinical pathological characteristics.Methods The expression of lgr5 at mRNA level was detected by SYBR Green quantitative real-time polymerase chain reaction (PCR) in 27 human colorectal cancer tissues and corresponding non-cancerous tissues as well as in peripheral blood of 17 patients and eight healthy controls.The differences of lgr5 mRNA expression in different tissues and clinical pathology parameters were analyzed by Wilcoxon test.Results The expression of lgr5 at mRNA level in colorectal cancer tissues was 1.000 (0.012,496.353),which was higher than that of corresponding non-cancerous tissues 0.147 (0.004,73.002),the difference was statistically significant (Z=8.029,P＜0.01).The lgr5 expression at mRNA level in peripheral blood of colorectal cancer patients was 0.742 (0.077,456.566),which was higher than that of healthy controls 0.104 (0.034,0.274) and the difference was statistically significant (Z=2.048,P＜0.05).There were no statistically significant differences between lgr5 expression at mRNA level and gender,age,primary location of tumor,tumor size and pathological type (all P＞0.05).However,the expression of lgr5 at mRNA level in group with lymph node metastasis was higher than that in group without lymph node metastasis (Z=2.066,P＜0 05).Conclusion The up-regulation of lgr5 gene expression in colorectal cancer tissues and peripheral blood may be involved in the growth and metastasis of colorectal cancer.
