Effect of endoplasmic reticulum stress on the activation of high glucose-induced monocytes

VernacularTitle:高糖刺激内质网应激诱导单核细胞活化
Author: Li HUANG ; Bing FENG ; Qi PANG ; Jigang CHEN ; Yanhong GUO ; Xiang DU ; Fahuan YUAN
Publication Type:Journal Article
Keywords: Endoplasmic reticulum stress; Monocyte; High glucose; Betaine
From: Chinese Journal of Nephrology 2013;(3):189-194
CountryChina
Language:Chinese
Abstract: Objective To observe the effects of endoplasmic reticulum stress (ERS) on the activation of monocytes induced by high glucose and explore the underlying mechanism.Methods The monocyte cell line THP-1 was stimulated with high glucose,and then treated with molecular chaperone betaine.The levels of glucose regulation protein 78 (GRP78) and p-JNK,which were associated with ERS were detected by real-time PCR and Western blotting.The proliferation of the cell line was detected by MTT method.Transwell and immunofluorescence were applied to observe the chemotaxis and phenotype of cells respectively.Results The levels of GRP78 and p-JNK of THP-1 cells stimulated by high glucose were significantly increased compared with the normal control group (all P ＜ 0.05).The proliferation and chemotactic were also enhanced (all P ＜ 0.05).The number of cells in M1 phenotype was increased remarkably (P ＜ 0.05).All the indexes above could be rescued by betaine.Conclusion The activation of THP-1 cells can be induced by high glucose through ERS,while molecular chaperone betaine can reverse the activation.