Effect of hepatitis B virus X gene on apoptosis and immune molecules of renal tubular epithelial cells
10.3760/cma.j.issn.1001-7097.2013.01.010
- VernacularTitle:乙型肝炎病毒X基因对肾小管上皮细胞凋亡及免疫分子的影响
- Author:
Xuan WANG
;
Yi ZHOU
;
Weijie YUAN
;
Nan ZHU
- Publication Type:Journal Article
- Keywords:
Transfection;
Hepatitis B virus;
Apoptosis;
Renal tubular epithelial cells;
Toll-like receptor 4;
Immune molecules
- From:
Chinese Journal of Nephrology
2013;(1):50-54
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of hepatitis B virus X (HBX) gene on apoptosis and immune molecules of human proximal renal tubular epithelial cell line (HK-2).Methods The eukaryotic vector pcDNA3.1-myc-HBX containing HBX gene was transiently transfected into HK-2 cells by lipofectamine mediation.Untransfected HK-2 cells and those transfected with empty vector were used as controls.The TLR4 expression was detected by real-time PCR and Western blotting.The apoptosis of cells and expression of MHC-Ⅱ and CD40 were detected by flow cytometry,and the contents of IL-4 and IFN-γ in the supernatant were detected by EIISA.Results Compared with control groups,the number of apoptotic cells was significantly increased in the HBX transfection group (P ＜ 0.05),and the expressions of TLR4,MHC-Ⅱ and CD40 were also significantly increased in the HBX transfection group (all P＜0.05).IFN-γ level in the supernatant of HBX transfection group was higher (P ＜ 0.05),but IL-4 level was lower as compared to control groups (all P ＜ 0.05).Conclusions Over-expression of HBX gene may induce apoptosis of HK-2 cells and upregulate the expression of immune molecules of renal tubular epithelial cells leading to injury of cells and dysfunction of immunomicroenviroment.
