Effects of electroacupuncture pretreatment on expression of phosphorylated signal transducer and activator of transcription 3 in cortical neurons in a rat model of focal cerebral ischemia/reperfusion injury
10.3760/cma.j.issn.0254-1416.2013.01.032
- VernacularTitle:电针预处理对局灶性脑缺血再灌注大鼠皮质神经元pSTAT3蛋白表达的影响
- Author:
Heng ZHOU
;
Haidong WEI
;
Feng WANG
;
Zijun GAO
;
Qiaomei ZHANG
;
Qiang WANG
;
Lize XIONG
- Publication Type:Journal Article
- Keywords:
Electroacupuncture;
Reperfusion injury;
Cerebral cortex;
STAT3 transcription factor
- From:
Chinese Journal of Anesthesiology
2013;(1):116-118
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of electroacupuncture (EA) pretreatment on the expression of phosphorylated signal transducer and activator of transcription 3 (pSTAT3) in cortical neurons in a rat model of focal cerebral ischemia/reperfusion (I/R) injury.Methods Forty-five adult male Sprague-Dawley rats,weighing 280-320 g,were randomly divided into 3 groups (n =15 each):sham operation group,I/R group and EA pretreatment group.Focal cerebral ischemia was induced by middle cerebral artery occlusion for 120 min,followed by 24 h of reperfusion.EA of Baihui acupoint lasting 30 min was performed and then the model of focal cerebral I/R was established 24 h later in EA group.Neurological function was assessed and scored at 24 h of reperfusion.The rats were then sacrificed and brains removed for detection of the cerebral infarct volume and expression of pSTAT3 (Ser727) in cortical neurons in ischemic penumbra by immunofluorescence and Western blot.Results Compared with sham operation group,the neurological function score was decreased,the infarct volume was increased,and the expression of pSTAT3 (Ser727) was up-regulated in groups I/R and EA (P ＜ 0.05).Compared with I/R group,neurological function score was increased,the infarct volume was decreased,and the expression of pSTAT3 (Ser727) was up-regulated in group EA (P ＜ 0.05).Conclusion EA pretreatment reduces focal cerebral I/R injury through up-regulating pSTAT3 expression in cortical neurons in rats.
