Changes in expression of Nogo-A protein in dorsal root ganglion and spinal dorsal horn in a rat model of inflammatory pain
10.3760/cma.j.issn.0254-1416.2013.01.018
- VernacularTitle:炎性痛大鼠背根神经节和脊髓背角Nogo-A蛋白表达的变化
- Author:
Danyang XU
;
Xuemei CHEN
;
Ying TANG
;
Licai ZHANG
- Publication Type:Journal Article
- Keywords:
Infammation;
Pain;
Spinal cord;
Ganglia,spinal;
Nogo-A protein
- From:
Chinese Journal of Anesthesiology
2013;(1):69-71
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the changes in the expression of Nogo-A protein in the dorsal root ganglion (DRG) and spinal dorsal horn in a rat model of inflammatory pain.Methods One hundred and twenty male Sprague-Dawley rats,weighing 250-300 g,were randomly divided into 2 groups (n =60 each):control group (group C) and formalin group (group F).The inflammatory pain was induced by injection of 3％ formalin 100 μl into the plantar surface of left hindpaw in group F.The equal volume of normal saline was given instead of formalin in group C.Mechanical withdrawal threshold to yon Frey filament stimulation was measured at 1 h and 1,2,3 and 7 days after injection.Twelve rats in each group were chosen at each time point and sacrificed.The L5 DRG and L4,5 segment of spinal cord on the operated side were removed for determination of Nogo-A protein expression by immunofluorescence and Western blot.Remlts Compared with group C,the mechanical withdrawal threshold was significantly decreased at 1 h,1,2,3 and 7 days after injection,and the expression of Nogo-A protein in the DRG and L4,5 segment of spinal cord was up-regulated at 1 h and 2,3 and 7 days after injection in group F (P ＜0.05).Conclusion Up-regulation of Nogo-A protein in the DRG and spinal dorsal horn may play an important role in the development of formalin-induced inflammatory pain in rats.
