Establishment of transgenic mouse models expressing human HSP22 protein
10.3760/cma.j.issn.0254-9026.2013.03.026
- VernacularTitle:表达人类小分子热休克蛋白22转基因小鼠模型的建立
- Author:
Fufeng ZHANG
;
Xiaoqin LU
;
Yafang ZHOU
;
Lu SHEN
;
Hong JIANG
;
Xinxiang YAN
;
Beisha TANG
- Publication Type:Journal Article
- Keywords:
Heat-shock proteins,small;
Mice,transgenic
- From:
Chinese Journal of Geriatrics
2013;(3):333-337
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish transgenic mouse models expressing human HSP22 protein.Methods pCAGGS-HA-Wt HSP22 transgenic expressing vector carrying human HSP22 gene was constructed by gene recombination technology.The linearized DNA was got by SalI、Hind Ⅲ and BsaⅪ digestion of PCAGGS-HA-Wt HSP22,purified and microinjected into fertilized eggs from C57BL mice.The tail DNA of pups was tested by PCR and DNA sequencing.Expression of human HSP22 protein was detected by western blot with anti-HA tag monoclonal antibody.Results 4 transgenic founder mice (Tg646,Tg648,Tg649,Tg661) carrying human HSP22 gene were identified by PCR and DNA sequencing.The human HSP22 protein was expressed in the lines Tg646,Tg648 and Tg649 founder mice,but was not expressed in the line Tg661 founder mouse.Conclusions The mouse models expressing human HSP22 protein are established successfully and provide the foundation for HSP22 gene research in vivo.
