PCR-based techniques for the detection of minority mutations
10.3760/cma.j.issn.1009-9158.2013.01.011
- VernacularTitle:基于PCR的少量突变检测技术的应用
- Author:
Wei WANG
;
Qishui OU
- Publication Type:Journal Article
- Keywords:
DNA Mutational analysis;
Polymerase chain reaction;
Transition temperature
- From:
Chinese Journal of Laboratory Medicine
2013;(1):40-45
- CountryChina
- Language:Chinese
-
Abstract:
With the development of molecular biology and molecular genetics,more attention has been taken to the advanced techniques which can be used to detect minority mutations.In recent years,many techniques such as amplification refractory mutation system,PCR clamping,pyrophosphorolysis-activated polymerization,co-amplification at lower denaturation temperature-PCR and digital PCR have been developed,which will make the hereditary disease diagnosis,cancer detection,noninvasive antenatal diagnosis step into a new stage in the near future.This review presents an overview of the principles,aoolications,advantages and disadvantages of these techniques.
