Association of Toll-like receptor 7 and Toll-like receptor 9 single nucleotide polymorphisms with chronic hepatitis C virus infection
10.3760/cma.j.issn.1674-2397.2013.02.006
- VernacularTitle:Toll样受体7和9基因多态性与慢性丙型肝炎病毒感染的相关性
- Author:
Xinsu WEI
;
Pingan ZHANG
;
Yongqing TONG
;
Chuandong WEI
;
Chengliang ZHU
- Publication Type:Journal Article
- Keywords:
Chronic hepatitis C;
Toll-like receptors;
Polymorphism,single nucleotide
- From:
Chinese Journal of Clinical Infectious Diseases
2013;(2):81-85
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the association of the single nucleotide polymorphism (SNP) of Toll-like receptor 7 (TLR7) and Toll-like receptor 9 (TLR9) with chronic hepatitis C virus (HCV)infection.Methods A total of 150 patients with chronic hepatitis C (CHC) admitted to Renmin Hospital of Wuhan University from January 2011 to May 2012 and 168 healthy controls were enrolled in the study.The genotypes of TLR7 IVS2-151 (rs179009) were detected by Sanger sequencing,and the genotypes of TLR9 T-1486C (rs187084) were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).SPSS 15.0 was used for statistical analysis,and goodness-of-fit test for HardyWeinberg equilibrium was also performed.Results The frequency of TLR7 IVS2-151G was higher in malepatients with CHC than that in male controls (41.4％ vs.21.6％,x2 =7.250,P =0.007,OR =0.389,95％ CI:0.194-0.781) ; however the female CHC patients had a higher frequency of TLR7 IVS2-151A than the female controls (76.9％ vs.63.1％,x2 =7.202,P =0.007,OR =1.942,95％ CI:1.192-3.164).No significant difference in the distribution of TLR9 T-1486C (rs187084) gene SNP was observed betweenCHC and control groups (P ＞0.05).Conclusion TLR7 IVS2-151 (rs179009) is correlated with HCV infection,which may be involved in the pathogenesis of CHC.
