Fabrication and application of a magnetic-targeting and controlled-release system using ST68-based microbubbles
10.3760/cma.j.issn.2095-2848.2013.01.005
- VernacularTitle:基于ST68微泡的磁靶向控释载体的制备及性能研究
- Author:
Zhanwen XING
;
Hengte KE
;
Jinrui WANG
;
Bo ZHAO
;
Enze QU
;
Xiuli YUE
;
Zhifei DAI
- Publication Type:Journal Article
- Keywords:
Ultrasonography;
Microbubbles;
Nanoparticles;
Superparamagnetic iron oxides;
Contrast media
- From:
Chinese Journal of Nuclear Medicine and Molecular Imaging
2013;(1):19-23
- CountryChina
- Language:Chinese
-
Abstract:
Objective To manufacture magnetic microbubbles with dual-response to ultrasound and magnetic fields.Methods Microbubbles of ultrasound contrast agent (ST68) based on a surfactant were prepared by the acoustic cavitation method.Fe3O4 magnetic nanoparticles with negative charge were synthesized using the polyol procedure.Magnetic microbubbles were generated by depositing polyethylenimine and Fe3O4 magnetic nanoparticles alternately onto the microbubbles using the layer-by-layer self-assembly.In vitro ultrasonography was performed on a silicone tube with/without magnetic microbubbles (3 × 108/ml) by a self-made device to observe the movement of magnetic microbubbles under the effects of magnetic field.In vivo imaging was performed on the kidney of New Zealand rabbits before and after the injection of magnetic microbubbles.Results The Fe3O4 nanoparticles carried a stable negative charge of (-24.6 ± 6.7) mV and more than 98％ of the particles were less than 8 μm in diameter,meeting the size requirement of an ultrasound contrast agent for intravenous administration.There was no echoic signal in the silicone tube before injection of magnetic microbubbles,but there were strong echoic signals after injection.After applying a magnetic field,the magnetic microbubbles moved along the direction of the magnetic flux.In vivo ultrasound imaging could not visualize the kidney before injection of magnetic microbubbles,but could remarkably visualize the kidney after injection.Conclusions The magnetic microbubbles exhibit favorable magnetic targeting and ultrasound contrast enhancement characteristics.Such properties may serve as the foundation to study their potential for simultaneous diagnosis and treatment in the future.
