Expression of apolipoprotein M in renal graft in rats and its roles and action mechanism in acute rejection
10.3760/cma.j.issn.0254-1785.2012.12.010
- VernacularTitle:大鼠肾移植后载脂蛋白M的表达变化及其在急性排斥反应中的作用和机制
- Author:
Hua TANG
;
Ying WAN
;
Chunmei ZHANG
;
Rong CHEN
;
Qingxi GUO
;
Weicheng ZENG
- Publication Type:Journal Article
- Keywords:
Rats;
Kidney transplantation;
Graft rejection;
Apolipoprotein M;
NF-κB
- From:
Chinese Journal of Organ Transplantation
2012;(12):737-741
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of apolipoprotein M in rats after renal transplantation and its role and action mechanism in acute rejection (AR).Methods The kidney transplantation model in rats were established.Male SD and Wistar rats were used as donors,and Wistar rats as recipients.Three groups were designated:control group (syngeneic transplantation,n =24,recipients were treated with normal saline i.p.); AR group (allogeneic transplantation,n =24,recipients were treated with normal saline i.p.); PDTC group [allogeneic transplantation,n =24,recipients were treated with NF-κB inhibitor-pyrrolidine dithiocarbamat (PDTC) i.p.].The renal grafts were drawn at day 1,3,5 and 7 post-transplantation,and the expression levels of NF-κB P65 and apoM protein were detected by using Western blotting,and those of apoM,perforin and granzyme B mRNA were by using real-time PCR.The correlation of apoM and NF-κB,apoM and perforin,and apoM and granzyme B was respectively analyzed.Results As compared with control group,the expression levels of apoM,perforin and granzyme B mRNA in AR and PDTC groups were dramatically up-regulated at each time point (P＜0.01),and those in PDTC group were significantly lower than those in AR group (P＜0.01).The expression levels of apoM and NF-κB protein in AR group were both distinctly higher than those in control group (P＜0.01),and those in PDTC group were markedly lower than those in AR group (P＜0.01).A significantly positive correlation was found between the expression of apoM and NF-κB protein (r =0.469,P＜0.05).And the expression of apoM mRNA was positively correlated to perforin and granzyme B mRNA (r =0.731,P＜0.01 ; r =0.514,P＜0.05).Conclusion The expression of apoM is obviously up-regulated in renal grafts of rats,which may take part in the pathogenesis of AR via NF-κB.
