Effects of ulinastatin pretreatment on oxidative stress response and hepatocyte growth factor after hepatectomy in rats
10.3760/cma.j.issn.0254-1416.2012.09.030
- VernacularTitle:乌司他丁预先给药对肝切除术大鼠氧化应激反应和肝细胞生长因子的影响
- Author:
Yulin ZHU
;
Ge ZHAO
;
Qining LIU
;
Rongsheng ZHOU
;
Xiaoqi ZHANG
;
Yi Lü
- Publication Type:Journal Article
- Keywords:
Trypsin inhibitors;
Hepatectomy;
Oxidative stress;
Hepatocyte growth factor
- From:
Chinese Journal of Anesthesiology
2012;(9):1136-1139
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of ulinastatin pretreatment on oxidative stress response and hepatocyte growth factor (HGF) after hepatectomy in rats.Methods One hundred and twelve pathogen-free male Sprague-Dawley rats,aged 3 months,weighing 230-280 g,were randomly divided into 2 groups (n=56 each): group hepatectomy (group H) and group ulinastatin pretreatment (group U).Left and median lobe resection was performed and then liver ischemia was induced by blood flow occlusion of right and caudate lobes for 30 min,followed by perfusion in both groups.Ulinastatin 50 000 U/kg was injected intravenously at 5 min before occlusion in group U.Eight rats in each group were chosen and the blood samples were taken from the inferior vena cava for measurement of serum ALT and AST activities and HGF concentration before ischemia and at 1,6,12,24and 48 h of reperfusion.Then the right lobe were removed for determination of apoptosis,SOD and myeloperoxidase (MPO) activities,MDA content,expression of proliferating cell nuclear antigen (PCNA) and liver regeneration.Apoptotic index was calculated.Another 8 rats in each group were chosen and the 7 day survival rate was recorded.Results Compared with group H,the levels of ALT,AST,MPO aud MDA and apoptotic index were significantly decreased,and the levels of HGF and SOD,PCNA expression and liver regeneration were significantly increased at different time points in group U (P ＜ 0.05).There was no significant difference in 7 day survival rate between group H and group U (P＞ 0.05).Conclusion Ulinastatin pretreatment can strengthen liver regeneration after hepatectomy in rats,the underlying mechanism may be related to inhibition of oxidative stress response and increase in HGF production.
