Effects of isoflurane on cell cycle and apoptosis in PC12 cells

VernacularTitle:异氟醚对PC12细胞株细胞周期与凋亡的影响
Author: Wenhui ZHAI ; Shuping HUO ; Qiujun WANG
Publication Type:Journal Article
Keywords: Isoflurane; Apoptosis; Cell cycle
From: Chinese Journal of Anesthesiology 2012;(9):1062-1064
CountryChina
Language:Chinese
Abstract: Objective To investigate the effects of isoflurane on the cell cycle and apoptosis in PC12 cells.Methods The neuronal PC12 cells were cultured for 7 d with nerve growth factor in vitro.The cells were cultured in 25 cm2 culture flask and randomly divided into 2 groups (n=6 each): control group (group C) and isoflurane group (group Ⅰ).The neuronal PC12 cells were exposed to 1.2％ isoflurane for 12 h in group Ⅰ.The cell morphology was examined by light microscopy.The apoptotic rate,cell cycle progression,mitochondrial membrane potential (MMP) and intracellular Ca2 + concentrations ([Ca2 +]i) were assessed by flow cytometry.Results Compared with group C,the cell morphology was changed,the proportion of the cells in Go/G1 phase and MMP were significantly decreased,and the proportion of the cells in G2/M phase,apoptotic rate and [Ca2+]i were significantly increased in group Ⅰ (P ＜ 0.05).Conclusion Exposure of PC12 cells to 1.2％ isoflurane for 12 h can activate the cell cycle abnormally and result in cell apoptosis.