Immunological function variation of peripheral dendritic cell from bladder cancer patients after blocking PD-L1 pathway
10.3760/cma.j.issn.1000-6702.2013.02.013
- VernacularTitle:阻断PD-L1通路对膀胱肿瘤患者外周血树突状细胞免疫功能的影响
- Author:
Xing LIU
;
Dengke YANG
;
Keqin ZHANG
;
Yanfeng LI
;
Yao ZHANG
;
Fengshuo JIN
- Publication Type:Journal Article
- Keywords:
Urinary bladder neoplasms;
Dendritic cells;
PD-L1 pathway blocking;
Immunomodulation
- From:
Chinese Journal of Urology
2013;(2):126-129
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the immunological function variation of peripheral dendritic cell(DC)from bladder cancer patients after blocking PD-L1 pathway.Methods DC from normal control and patient with bladder cancer were cultured with rhGM-CSF,rhIL-4 in vitro and treated with PD-L1 monoclonal antibody.The expression of CD1a,HLA and CD83 were examined by flow cell meter.The effect of DC induced lymphatic cellular proliferation and its capability of secreting IL-10,IL-12 were determined by MTT and ELISA.Results Blocking PD-LI pathway did not infuluence the maturation of DC.But the DCs from bladder cancer patient signifcantly boosted the lymphatic cellular proliferating(4.00 ± 1.28 versus 1.49 ±0.45)and IL-12 secretion capability(108.30 ± 21.89 versus 37.17 ± 14.89 ng/L),and yet it also decreased the secretion of IL-10(108.90 ± 21.77 versus 14.99 ± 54.99 ng/L)after blocking PD-L1 pathway (P ＜ 0.05).Conclusion Blocking PD-LI pathway on the DC from bladder cancer patient may improve its anticancer immunological function.
