Expression and role of miR-34a in bladder cancer
10.3760/cma.j.issn.1000-6702.2012.12.017
- VernacularTitle:微小核糖核酸-34a在膀胱癌组织中表达的临床意义
- Author:
Wei WANG
;
Tan LI
;
Lihua SHI
;
Ying LI
;
Hui LI
- Publication Type:Journal Article
- Keywords:
MicroRNA-34a;
Bladder neoplasm;
Carcinoma;
Apoptosis
- From:
Chinese Journal of Urology
2012;(12):939-942
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression and biological function of microRNA-34a (miR-34a) in bladder cancer.Methods Forty-two cases of bladder cancer specimen,including 17 noninvasive carcinoma and 25 muscle invasive carcinoma classified by UICC 2002 TNM,or 18 low-grade and 24 high-grade classified by WHO 1973.Meanwhile,mucosa adjacent to the carcinoma was selected as normal control.The gene expression of miR-34a was determined using real-time quantitative polymerase chain reaction in 42 cases of bladder carcinoma samples.Mature mimics of miR-34a were chemically synthesized and transiently transfected-intoT24 bladder cancer cells.The effects of miR-34a on apoptosis and proliferation in T24 cells were evaluatedby flow cytometry and MTS respectively.Results 61.9％ of carcinoma samples showed low expression of miR-34a,which was correlated with the malignancy and tumor size of bladder carcinoma.26.5％ (11 cases) was negative and 11.6％ (5 cases) showed high expression.Furthermore,upregulation of miR-34a in T24 cells contributed to cell growth and apoptosis.The apoptosis rate of T24 cells was (9.11 ± 0.41)％,which had significant difference compared with NC mimics and blank control group respectively (P ＜ 0.01).Conclusion The relative low expression of miRNA-34a may be involved in the tumorigenesis and development of bladder carcinoma.
