Influence of estrogen receptor a on proliferation and metastasis in bladder cancer cells
10.3760/cma.j.issn.1000-6702.2012.11.008
- VernacularTitle:雌激素受体α对膀胱癌细胞增殖和转移影响的实验研究
- Author:
Zhiqun SHANG
;
Minghao ZHANG
;
Libin SUN
;
Qiliang CAI
;
Ning JIANG
;
Ruifa HAN
;
Yuanjie NIU
- Publication Type:Journal Article
- Keywords:
Urinary bladder neoplasms;
Carcinoma;
Estrogen receptor alpha;
Cell proliferation;
Neoplasm metastasis
- From:
Chinese Journal of Urology
2012;(11):827-830
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the function and mechanism of estrogen receptor α (ERα) in bladder cancer cell proliferation and aggressivity.Methods The ERα expression bladder cancer cell line T24ERα model was established.The cell growth was detected by MTT assay,apoptosis by flow cytometry,cell invasion by matrigel transwell.Western blot was used to check signals by ERα regulation in bladder cancer cells related to the proliferation and metastatic ability.Results Compared to the control group,the cell inhibition rates of experimental group in 96 h and 144 h were 18.85％ and 37.21％,respectively.The difference was significant compared with the control group (P ＜ 0.05).The apoptosis rates of the experimental group and control group were (18.93 ±1.41)％ and (9.91 ±1.08)％ (P＜0.05).The experimental group through matrix adhesive cell proportion was (10.00 ± 2.00)％,significantly lower than that of the control group (26.00 ± 3.61) ％ (P ＜ 0.05).Western blot showed integrin-β1,p-FAK,p-Src and Scr expression were reduced compared to control group (P ＜ 0.05).Conclusion ERα could inhibit bladder cancer cell growth and metastasis through down-regulating integrin-β1-FAK/Src signal pathway,while promote the apoptosis of bladder cancer cells.
