Effect of apolipoprotein E gene polymorphism on early apoptosis of astrocytes after hypoxic injury
10.3760/cma.j.issn.1001-8050.2013.02.019
- VernacularTitle:载脂蛋白E基因多态性在星形胶质细胞缺氧性损伤后早期凋亡中的作用
- Author:
Shuai ZHOU
;
Li JIANG
;
Chongjie CHENG
;
Cheng YIN
;
Xiaochuan SUN
- Publication Type:Journal Article
- Keywords:
Apolipoprotein E;
Polymorphism,single nucleotide;
Astrocytes;
Mitochondria
- From:
Chinese Journal of Trauma
2013;(2):170-174
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate effect of polymorphism of apolipoprotein E (APOE) gene on early apoptosis of astrocytes after hypoxic injury.Methods Astrocytes separated from APOE wild mice and APOE transgenic mice (ε3,ε4) were primarily cultured,and then purified and identified.Models of astrocyte hypoxic injury were set up by hypoxia.Morphological changes of astrocytes and mitochondria were observed by transmission electron microscope.Early apoptosis rate and changes of mitochondrial membrane potential were detected by flow cytometry.Results Cell foot process tumidness and mitochondria with irregular outline,vacuoles and irregular cristae were observed in each group by electron microscopy at six hours after hypoxia.There were no significant differences of cellular form changes among groups.Early apoptosis rate and decreasing degree of mitochondrial membrane potential in APOFε4 group were significantly higher than those in APOEε3 group and APOE wild group (P ＜ 0.05).Conclusion Compared with astrocytes from APOEε3 group and APOE wild group,mitochondrial membrane potential in astrocytes from APOEε4 group at early period after hypoxia declines more significantly,as may be one of causes for more astrocyte apoptosis.
