Expression alteration of caspase-12 and demyelination after compressed spinal cord injury

VernacularTitle:脊髓压迫性损伤后脱髓鞘病变及caspase-12表达变化
Author: Siqin HUANG ; Wei QI ; Shanquan SUN ; Kejian WANG ; Fei ZHUO ; Weitian LU
Publication Type:Journal Article
Keywords: Spinal cord injuries; Demyelinating diseases; Oligodendroglia; Caspase 12; Apoptosis
From: Chinese Journal of Trauma 2013;(2):160-164
CountryChina
Language:Chinese
Abstract: Objective To investigate correlation between demyelination and caspase-12 expression alteration after compressed spinal cord injury (CSCI) so as to discuss mechanism of demyelinating lesion after CSCI.Methods Seventy-five adult SD rats were randomly divided into five groups,ie,normal group,control group,compression 1 d,3 d and 7 d groups,with 15 rats per group.Models of spinal cord compression were established with a self-made device.Ultrastructure of the demyelinated nerve fibers was observed by electronic microscope and oligodendrocyte apoptosis was detected by TUNEL staining and double labeling immunofluorescence.Immunoblotting was used to defect caspase-12 that was related to cell apoptosis.Results Demyelination of nerve fiber occurred after CSCI and was aggravated with time.Apoptosis of oligodendrocytes was found after CSCI,and showed significant difference between compression 7 d group and normal group (P ＜ 0.05).Caspase-12 was also upregulated with extension of compression time.Conclusion Caspase-12 mediating oligodendrocyte apoptosis is one of the mechanisms of nerve fiber demyelination after CSCI.