Regulative effect of substance P on proliferation of human skin fibroblast and expression of its monocyte chemoattractant protein-1 under high glucose condition
10.3760/cma.j.issn.1001-8050.2012.12.020
- VernacularTitle:P物质对高糖条件下成纤维细胞增殖及其单核细胞趋化蛋白-1合成的调节作用
- Author:
Zhigang JIA
;
Yong FANG
;
Min YAO
;
Tao NI
;
Xiaoguang LI
;
Youzhen SHI
- Publication Type:Journal Article
- Keywords:
Substance P;
Fibroblasts;
Monocyte chemotactic protein;
Wound healing
- From:
Chinese Journal of Trauma
2012;(12):1125-1129
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate effects of exogenous substance P (SP) on proliferation of humau skin fibroblast (HSF) and expression of its monocyte chemoattractant protein-1 (MCP-1) under high glucose condition.Methods HSF cultured in high glucose DMEM medium were treated with SP of various concentrations at different time points.Levels of MCP-1 in the supernatants were determined by ELISA and time-effect relationship of SP regulating expression of MCP-1 was analyzed.Expression of MCP-1 mRNA in HSF was detected by semi-quantitative RT-PCR and Real Time-PCR,Conclusion,while cxpression of MCP-1 by Western blot.Proliferation of HSF treated with SP of different concentrations was detected by cell counting Kit-8 (CCK-8).Results MCP-1 had a strong expression at 8 hours after SP disposal (P ＜0.05) and reached the peak at 24 hours (P ＜ 0.01).Meanwhile,MCP-1 expressed the most at 10 nmol/L and 100 nmol/L SP (P ＜0.01).SP significantly enhanced the expression of MCP-1 mRNA and its synthesis under high glucose condition,especially at the concentration of 10 nmol/L (P ＜0.01).Also,SP induced obvious proliferation of HSF at concentrations of 10 nmol/L and 1 000 nmol/L(P ＜ 0.05).Conclusion SP promotes proliferation of HSF and expression of MCP-1 in high glucose DMEM medium,which may be of significance in promoting diabetic wound healing.
