Effects of transplanting bone marrow stem cells transfected with bone morphogenetic protein-2 on fracture healing in diabetic rats
10.3760/cma.j.issn.1001-8050.2012.11.021
- VernacularTitle:骨形态发生蛋白-2基因转染骨髓间充质干细胞移植对糖尿病大鼠骨折愈合的影响
- Author:
Deliang WANG
;
Deguo XING
;
Jianjun WU
;
Liang WANG
;
Keli TIAN
;
Zhonghao LIU
;
Mingzhi GONG
- Publication Type:Journal Article
- Keywords:
Myeloid progenitor cells;
Bone morphogenetic proteins;
Diabetes mellitus;
Gene transfer techniques
- From:
Chinese Journal of Trauma
2012;(11):1042-1045
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of transplantation of bone marrow stem cells(BMSCs)transfected with bone morphogenetic protein-2(BMP-2)on fracture healing in rats with diabetes so as to provide a new therapy for diabetic fractures.Methods Fifty male adult Wistar rats aged six weeks randomized to the control group and experimental group were all employed to establish models with diabetic fractures.Under high glucose condition,BMSCs were transfected with BMP-2 by adenovirus vector in vitro.BMSCs transfected by BMP-2 were transplanted into the fracture area of rats in the experimental group,while non-transfected BMSCs into the corresponding area of rats in the control group.X-ray examination was performed at 1,2,3,4 and 6 weeks after transplantation.Bony calluses were collected for HE staining and gray scales of BMP-2 in calluses were determined by immunohistochemical method.Meanwhile,serum levels of BMP-2 were measured by ELtSA.Results The gray scales of BMP-2 in the calluses were 83±3 in the experimental group and 118±4 in the control group at four weeks(P＜0.01).The serum concentrations of BMP-2 were(203.80±8.96)ng/L in the experimental group and(139.15±4.19)ng/L in the control group at four weeks(P＜0.01).Conclusion Transplantation of BMSCs transfected by BMP-2 promotes fracture healing in diabetic rats.
