Effects of rosiglitazone on interlukin-6 and interlukin-10 levels in lung tissue of diabetic rats
10.3760/cma.j.issn.1008-6315.2013.03.001
- VernacularTitle:罗格列酮对糖尿病大鼠肺组织中白细胞介素6和白细胞介素10表达的影响
- Author:
Liping NIE
;
Weixin MU
;
Yaxin SHEN
;
Qingjun GUO
- Publication Type:Journal Article
- Keywords:
Rosiglitazone;
Diabetes mellitus;
Interlukin-6;
Interlukin-10
- From:
Clinical Medicine of China
2013;(3):225-228
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of rosiglitazone on the concentration of interlukin (IL)-6 and IL-10 in lung tissues of diabetic rats.Methods The experimental diabetic rats were yielded by injecting streptozotocin(STZ) and feeding with high fat and high glucose food.We observed lung morphology in control group,diabetes mellitus(DM) group,and rosiglitazone group at 10 week and 20 week respectively under light microscope.Alteration of IL-6 and IL-10 in lung was measured by immunohistochemistry.Results The optical density values of IL-6 in the control group,the DM group and the roggerosiglitazone treatment group were 0.15 ±0.01,0.16 ±0.01;0.22 ±0.02,0.31 ±0.04;0.22 ±0.03,and 0.20 ±0.02 at 10 week and 20 week respectively (Fwithin =216.89,P ＜ 0.01 ; Fbetween =342.62,P ＜ 0.01 ; Finteraction =341.51,P ＜ 0.01).Any two groups had significant difference(P ＜ 0.05) except the comparison of the IL-6 values at 10 week and 20 week in the control group (P ＞ 0.05).The absorbance values of IL-10 in the three groups were 0.13 ± 0.01,0.15 ±0.02;0.20 ±0.01,0.21 ±0.01;0.20 ±0.02,and 0.17 ±0.01 at 10 week and 20 week respectively (Fwithin =14.612,P ＜0.01 ;Fbetween =909.19,P ＜0.01 ;Finteraction =210.55,P ＜0.01).Any two groups had significant difference(P ＜0.05) except the comparison of the IL-6 values at 10 week and 20 week in the control group.Conclusion The elevated levels of IL-6 and IL-10 in lung tissue of dibtetic rats might be related to the inflammation of lung tissues.Rosiglitazone may alleviate lung inflammation by regulating the levels of IL-6 and IL-10.
