Simultaneous determination of benzoic acid and salicylic acid in Compound Benzoic acid tincture by RP-HPLC

VernacularTitle:反相高效液相色谱法同时测定复方苯甲酸碘酊中苯甲酸和水杨酸的含量
Author: Liju ZHANG ; Wuxi ZHAO
Publication Type:Journal Article
Keywords: Compound Benzoic acid tincture; RP-HPLC; Benzoic acid; Salicylic acid
From: Chinese Journal of Primary Medicine and Pharmacy 2012;(22):3373-3374
CountryChina
Language:Chinese
Abstract: Objective To estabilish a RP-HPLC method for simultaneous determination of benzoic acid and salicylic acid in Compound Benzoic acid tincture.Methods ODSC18 chromatographic column made from Dalian Yilite(4.6mm × 150mm,5μm);mobile phase:methanol: water=60|: 40(pH adjust to 3.2);column temperature:30℃;detection wavelength:242nm;fiow rate:0.9ml/min.Results Ranges of linear of benzoic aci and salicylic acid were 30～120mg/L(R2=0.9995)and 15～60mg/L(R2=0.9988).The average recovery rate was 99.2％(RSD=0.95％)and 99.1％(RSD=1.36％).The RSDs of intra-day were 1.57％ and 1.57％(n=5).The BSDs of interday were 1.43％ and 1.59％(n=5).With t test,difference between Acid-base titration and RP-HPLC was not significant(P＞0.05).Conclusion The metold is simple,accurate and sensitive and can be used for the quality control of Compound Benzoic acid tincture.