An in vitro study of thrombomodulin-protein C-EPCR system coagulation function in hemorrahgic shock rats
10.3760/cma.j.issn.1671-0282.2012.12.013
- VernacularTitle:失血性休克大鼠体外实验关于内皮细胞蛋白C系统的研究
- Author:
Ye SHEN
;
Wenwei CAI
;
Shuanghua WU
;
Meiqi ZHANG
;
Jingye PAN
- Publication Type:Journal Article
- Keywords:
Hemorrhagic shock;
Thrombomodulin;
Endothelial cell protein C receptor;
Coagulation disorders;
Polymerase chain reaction
- From:
Chinese Journal of Emergency Medicine
2012;(12):1354-1357
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the coagulant function of Thrombomodulin-Protein C-EPCR System in rats with hemorrhagic shock in virto study.Methods In this study,30 rats were divided into three groups,with each group 10 rats:control group,the sham shock group,hemorrhagic shock group.Hemorrhagic shock model was subjected to computer-controlled arterial hemorrhage to 40 mm Hg for 60 min The rat blood serum samples were obtained from rats in sham shock group and shock group after maintaining 3 hours,and fetal bovine serum (FBS) was used as control group.Then medium contained fore-mentioned serums cultured with SV40-transformed aortic rat endothelial cells for 6 hours,12 hours and 18hours.The messenger RNA (mRNA) expression of thrombmodulin (TM),Endothelial cell protein C receptor in the SV40-transformed aortic rat endothelial cells which were treated with serums were detected by reverse-transcription polymerase chain reaction.Results Compared to control group,SVARECs were incubated for 6hrs with shock serum,the mRNA expression of TF,TM and t-PA was significantly higher,and continued elevating at the time of 18 hours (P ＜ 0.01).Conclusions Serums from hemorrhagic shock rats upregulate TMmRNA EPCRmRNA expression on the endothelial cells surface.It is suggested that Thrombomodulin-Protein C-EPCR System pay a key role of anticoagulation,the protection of endothelial function.
