The effect of Gαi2 on neuronal apoptosis in cerebral ischemia-reperfusion injury model of rats
10.3760/cma.j.issn.1671-0282.2012.12.002
- VernacularTitle:Gαi2对脑缺血-再灌注后神经细胞凋亡的影响
- Author:
Jian LI
;
Peng LIU
;
Yiliu LIAO
- Publication Type:Journal Article
- Keywords:
Gαi2;
Cerebral ischemia reperfusion injury;
Hippocampus;
Pertussis toxin;
Apoptosis;
Neuron;
Ca2+;
Death
- From:
Chinese Journal of Emergency Medicine
2012;(12):1303-1307
- CountryChina
- Language:Chinese
-
Abstract:
Objective To discuss the levels of inhibitory protein Ga-2 (Gαi2) in hippocampus of brain and the effects of Gαi2 on neuronal intracellular Ca2+ level in cerebral ischemia reperfusion injury model of rats.Methods Ninety SD rats were randomly (random number) assigned to sham group (n =30),ischemic-reperfusion (IR) group (n =30),Pertussis toxin (PT) group (n =30).The blood flow of right common carotid artery of rat was blocked for 90 min to make ischemia reperfusion model.The levels of Gαi2 in hippocampus was assayed by immunohistochemistry and Western blotting after ischemia reperfusion for 6,12,24 h in each group.The average fluorescence values of intracellular Ca2+ levels in hippocampus of rats in three groups were detected by using Flow CytoMeter (FCM).Neuronal cell apoptosis was measured by TUNEL.Results After restoration of middle cerebral artery blood flow for different lengths of time,the levels of hippocampus Gαi2 and Ca2+ levels in IR group were significantly higher than those in Sham group (P ＜0.01).The hippocampus Ca2+ levels in PT group were higher than those in IR group (P ＜ 0.01).The apoptotic rates of neurons in PT group were lower than those in IR group (P ＜0.05).Conclusions The level of Gαi2 in cerebral ischemia reperfusion injury model was increased.Gαi2 might reduce the calcium ion concentration of neurons after cerebral ischemia and rcduce the neuronal cell apoptosis in this model.Gαi2 might play a role in protecting neuron from cerebral ischemia reperfusion injury.
