Study of nanofiber microcarriesr/collagen gel on in vitro cultivation of hepatocytes
10.3760/cma.j.issn.1673-4181.2012.06.001
- VernacularTitle:纳米纤维微载体/胶原凝胶复合体系体外肝细胞培养的研究
- Author:
Wei HU
;
Xin LIU
;
Hanqing GU
- Publication Type:Journal Article
- Keywords:
Nanofiber microcarrier;
Hepatocytes;
Collagen gel;
Cell culture;
Bioartificial liver
- From:
International Journal of Biomedical Engineering
2012;(6):321-324
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate cell's viability and functions when human hepatocyte cell HepG2 and microcarriers were embedded in collagen gel and cultured in vitro.Methods Ultrasound concussion crushing method was used to get nanofiber microcarrier.HepG2 and microcarriers were embedded in collagen gel and cultured in 12 d.The albumin (ALB),urea and lactate dehydrogenase (LDH) levels in the nutrient solution were measured.Results The cells in nanofiber nanofiber microcarrier/collagen gel group were gathered around the microcarrier and formatted aggregates,the ALB and urea levels increased steadily and reached maximum in day 9,then decreased; In collagen gel without nanofiber microcarrier group cells uniformly distributed,the ALB and urea levels reached maximum in day 3,then decreased rapidly,and on day 6 majority of cells dead.Conclusion Gel entrapped nanofiber microcarriers and hepatoeytes is a high-density and longtime culture method which can possibly be used in the bioartificial liver system.
