Development of a real-time SYBR Green PCR assay for the rapid detection of Dermatophilus congolensis.
10.4142/jvs.2013.14.4.491
- Author:
Alfredo GARCIA
1
;
Remigio MARTINEZ
;
Jose Manuel BENITEZ-MEDINA
;
David RISCO
;
Waldo Luis GARCIA
;
Joaquin REY
;
Juan Manuel ALONSO
;
Javier Hermoso DE MENDOZA
Author Information
1. Departamento de Sanidad Animal, Facultad de Veterinaria de Caceres, Universidad de Extremadura, 10003 Caceres, Spain. alfrgcia@unex.es
- Publication Type:Brief Communication ; Evaluation Studies ; Research Support, Non-U.S. Gov't
- Keywords:
clinical samples;
Dermatophilus congolensis;
dermatophilosis;
diagnosis;
real-time PCR
- MeSH:
Actinomycetales/*isolation & purification;
Actinomycetales Infections/diagnosis/microbiology/*veterinary;
Animals;
Cattle;
Cattle Diseases/*diagnosis/microbiology;
Fluorescent Dyes/*diagnostic use;
Horse Diseases/*diagnosis/microbiology;
Horses;
Limit of Detection;
Real-Time Polymerase Chain Reaction/*methods/veterinary;
Reproducibility of Results;
Sheep;
Sheep Diseases/*diagnosis/microbiology
- From:Journal of Veterinary Science
2013;14(4):491-494
- CountryRepublic of Korea
- Language:English
-
Abstract:
Methods such as real time (RT)-PCR have not been developed for the rapid detection and diagnosis of Dermatophilus (D.) congolensis infection. In the present study, a D. congolensis-specific SYBR Green RT-PCR assay was evaluated. The detection limit of the RT-PCR assay was 1 pg of DNA per PCR reaction. No cross-reaction with nucleic acids extracted from Pseudomonas aeruginosa, Mycobacterium tuberculosis, Staphylococcus aureus, or Austwickia chelonae was observed. Finally, the RT-PCR assay was used to evaluate clinical samples collected from naturally infected animals with D. congolensis. The results showed that this assay is a fast and reliable method for diagnosing dermatophilosis.
