Primary culture and epithelial differentiation of human adipose-derived stem cells
10.3760/cma.j.issn.1671-0290.2012.06.013
- VernacularTitle:脂肪干细胞原代培养及其向表皮细胞诱导分化的研究
- Author:
Qiang LI
;
Peisheng JIN
;
Aijun ZHANG
;
Changbo TAO
;
Xueyang LI
;
Jing WANG
- Publication Type:Journal Article
- Keywords:
Adipose-derived stem cells;
Cell differentiation;
Epidermal cells;
Wound healing
- From:
Chinese Journal of Medical Aesthetics and Cosmetology
2012;(6):440-443
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the possibility of primary culture of human adipose-derived stem cells in vitro and differentiation induction into epidermal cells.Methods Adipose-derived stem cells were primarily cultured by enzyme digestion method.The expression of CD29,CD34,CD44,CD49d,CD80 and CD106 was detected by immunohistochemical staining.ADSCs differention into epidermal cells in vitro were under induction medium.Proliferation activity and morphology of cells of induction group and control group were observed,and the expression of CKs in the two groups were also analyzed.Results ADSCs were successfully isolated and cultured from human liposuction tissue.It was revealed by immunofluorescence staining that ADSCs possessed specified expression of surface antigen of stem cells; ADSC successfully differentiated towards epidermal cells in vitro and possesed considerable high-level reproductive activity,cell morphology and expression of CKs also shown the tendency of differentiated into epidermal cells.Conclusions ADSCs can be isolated and cultured from human liposuction tissue,and can proliferate persistently.ADSCs possess specified expression of surface antigen.ADSCs can also be differentiated in vitro to the direction of epidermal cells.
