Effect of multiple IPL treatment on cell senescence markers in skin fibroblasts
10.3760/cma.j.issn.1671-0290.2012.05.014
- VernacularTitle:多次强脉冲光照射对皮肤成纤维细胞老化指标的影响
- Author:
Ruiyan WANG
;
Wei LIU
;
Xiaozhong ZHAO
;
Xiaoying YUAN
;
Hongmei LIU
- Publication Type:Journal Article
- Keywords:
Intense pulsed light (IPL);
Senescence-associated β-galactosidase (SA-β-Gal);
Cell cycle;
Reactive oxygen species (ROS);
Telomere length;
Ultraviolet A
- From:
Chinese Journal of Medical Aesthetics and Cosmetology
2012;(5):364-367
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of multiple IPL treatment on cell senescence markers of skin fibroblasts using UVA as a control and to make clear whether the multiple IPL treatment may result in cell senescence.Methods Cells were divided into three groups: one group without irradiation as a control,one group receiving IPL treatment with 15 J per cm2,and the last group receiving UVA irradiation with 9 J per cm2.IPL and UVA irradiation were performed once a day during five days.On the sixth day,the cells were collected.Senescence-associated β-galactosidase (SA-β-Gal) staining,cell cycle,reactive oxygen species (ROS) and telomere length were determined.Results Our results showed that five consecutive days of IPL irradiation had no effect on the activity of SA-β-Gal and telomere length and decreased the G1 ％ of cell cycle and the level of ROS in comparison with the control group (P＜0.05).On the contrary,five consecutive days of UVA irradiation increased the activity of SA β Gal and the level of ROS,shortened the length of telomere and no obvious change in the G1 ％ of cell cycle in comparison with the control group.Conclusions Multiple UVA irradiations induce cell senescence.On the contrary,multiple IPL treatments could not induce cell senescence.
