An experimental study of the impacts of magnetic fields on superoxide dismutase and malondialdehyde in rat pancreatic islet cells
10.3760/cma.j.issn.0254-1424.2012.010.004
- VernacularTitle:不同强度永磁磁场对大鼠胰岛细胞超氧化物歧化酶及丙二醛的影响
- Author:
Lin YANG
;
Yimin WANG
;
Qingnan MENG
;
Yanni MENG
;
Haonan ZHANG
;
Yunhua WANG
- Publication Type:Journal Article
- Keywords:
Magnetic fields;
Islet cells;
Oxidative damage
- From:
Chinese Journal of Physical Medicine and Rehabilitation
2012;(10):733-736
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the impacts of magnetic fields of different intensities on the superoxide dismutase (SOD) activity and malondialdchvde (MDA) levels in rat pancreatic islet cells under normal and hypoxic conditions.Methods Rat pancreatic islet cells were culured,and after 3 days were subjected to a magnetic field of either 44.8 mT,90.6 mT or 182.1 mT under either normal or hypoxic conditions.Control cells received no magnetic field exposure,SOD activity and MDA level were measured after 72 hr.Results The cultured cells grew linearly with optical density (OD) of 0.067 ± 0.021 after 2 days and 0.449 ± 0.113 afier 5 days.SOD activity was significantly lower in the three magnetic field intervention groups than in the control group.Under hypoxic culture conditions,in all the magnetic field intervention groups SOD activity increased at first and then deereased.Under normal culture conditions,MDA content was significantly higher in the 182.1 mT group than in the control group.In the other two groups it was significantly lower.Conclusion Magnetic field exposure can cause oxidative damage to pancreatic islet cells,at least rat cells in culture.Under hypoxic culture conditions a magnetic field can inhibit such damage.
