The effect of transcranial magnetic stimulation on the proliferation of neural stem cells and miR-9 in the brain after focal cerebral ischemia
10.3760/cma.j.issn.0254-1424.2012.010.002
- VernacularTitle:重复经颅磁刺激对脑缺血后神经干细胞增殖以及miR-9表达的影响
- Author:
Jinghui ZHANG
;
Xiaohua HAN
;
Xiuxiu ZHAO
;
Feng GUO
;
Hua LIU
;
Xiaolin HUANG
- Publication Type:Journal Article
- Keywords:
Transcranial magnetic stimulation;
Ischemia;
Neural stem cells;
MicroRNAs
- From:
Chinese Journal of Physical Medicine and Rehabilitation
2012;(10):725-728
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of repetitive transcranial magnetic stimulation (rTMS) on the proliferation of neural stem cells in the subventricular zone (SVZ) and the expression of miR-9 in the ipsilesional hemisphere of rats with focal cerebral ischemia.Methods A rat model of acute focal cerebral ischemia was established using transient middle cerebral artery occlusion (tMCAO).Rats were then randomly assigned to a sham group,a control group or an rTMS group.Daily rTMS treatments (10 trains with 30 pulses per train and 50-second breaks between trains at 120％ of the resting motor threshold) were targeted at the ipsilesional MI cortex beginning one day after the tMCAO.After the treatment the proliferation of neural stem cells in the ipsilesional SVZ was identified by double immunofluorescence staining (BrdU/nestin).The expression of miR-9 was evaluated using quantitative PCR.Results Compared with the other two groups,BrdU + Nestin + cells in the ipsilesional SVZs of the rTMS group increased and the expression of miR-9 decreased significantly.Conclusion rTMS can promote the proliferation of neural stem cells and inhibit the expression of miR-9 in the ipsilesional hemisphere after focal cerebral ischemia.
