Effect of globular domain of adiponectin on glucose metabolism and insulin signal transduction in insulin resistant model adipocytes

VernacularTitle:脂联素球状结构域对胰岛素抵抗模型脂肪细胞及胰岛素信号转导的影响
Author: Yan WANG ; Sisi CHEN ; Ru LIAN ; Xianjiu CHEN ; Jing YANG
Publication Type:Journal Article
Keywords: Globular domain of adiponectin; 3T3-L1 adipocytes; Insulin signal transduction; Insulin resistant
From: Chinese Journal of Endocrinology and Metabolism 2012;(12):1016-1019
CountryChina
Language:Chinese
Abstract: Insulin resistance model of 3T3-L1 adipocytes were prepared with plamotic acid.Adipocytes with generated insulin resistance were cultured with different concentrations of globular domain of adiponectin(gAd:250,500,1 000 ng/ml).The cell culture medium glucose content was detected with the glucose oxidase method,the mRNA expressions of insulin receptor substrate-1 (IRS-1),phosphatidylinositol-3 kinase(PI3K),and protein kinase B(PKB) were detected with real-time quantitative PCR method.The phosphorylation of IRS-1 was detected by Western blot.Compared with the control group,the experimental group showed significantly increased glucose consumption (P ＜ 0.01),and with the increasing gAd concentration,glucose consumption was gradually increasing.IRS-1 phosphorylation was increased gradually with the increasing concentration of gAd.These results suggest that gAd can promote glucose uptake by 3T3-L1 adipocyte model with generated insulin resistance.This may be correlated with promoting insulin signal transduction and improving insulin resistance in adipocytes.