Effect of metformin on proliferation and related genes expression of human osteoblast MG63 under high glucose

VernacularTitle:二甲双胍对高糖介导的成骨细胞MG63增殖及相关基因表达的影响
Author: Xiaojun CAO ; Bimin SHI ; Xuan DU ; Yongjing CHEN
Publication Type:Journal Article
Keywords: Metformin; Osteoblasts; Proliferation; Matrix metalloproteinases
From: Chinese Journal of Endocrinology and Metabolism 2012;(12):972-974
CountryChina
Language:Chinese
Abstract: The proliferation of MG63 cells under high glucose intervened with metformin was measured by CCK-8 assay.The activity of intracellular alkaline phosphatase (ALP) was detected by SFBC assay.The expression of related genes was detected by RT-PCR.Metformin promoted the proliferation of MG63 cells (P＜0.01),the activity of ALP was increased (P＜0.05).The expressions of collagen Ⅰ and osteocalcin were up-regulated while the expressions of matrix metalloproteinase (MMP)-1,MMP-2,MMP-3,and MMP-8 were down-regulated (P＜0.05).The results indicate that metformin may protect osteoblast,via promoting osteoblast proliferation,differentiation and mineralization,as well as by inhibiting degradation of osteoblast extracellular collagen matrix.