Effect of sevoflurane anesthesia on cognitive function and phosphorylation of tau in hippocampal neurons in amyloid precursor protein transgenic mice
10.3760/cma.j.issn.0254-1416.2012.12.005
- VernacularTitle:七氟醚麻醉对淀粉样前体蛋白转基因小鼠认知功能及海马神经元tau蛋白磷酸化水平的影响
- Author:
Changsheng LI
;
Sufang LIU
;
Shuaiguo Lü
;
Yu BAI
;
Yalin SUN
;
Yanping FENG
- Publication Type:Journal Article
- Keywords:
Anesthetics,inhalation;
Cognition disorders;
tau Proteins;
Alzheimer disease
- From:
Chinese Journal of Anesthesiology
2012;(12):1433-1436
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of sevoflurane anesthesia on the cognitive function and phosphorylation of tau in hippocampal neurons in amyloid precursor protein (APP) transgenic mice.Methods Male APP gene mutation mice,weighing 18-22 g,aged 8-12 weeks,were used in the study.Forty-four APP positive mice were randomly divided into 2 groups (n =10 each):sevoflurane group (group AS,n =28) and control group (group AC,n =16).Forty-four APP negative mice were randomly divided into 2 groups:sevoflurane group (group S,n =28) and control group (group C,n =16).Animals in groups S and AS inhaled 3％ sevoflurane for 4 h.While in groups C and AC,animals inhaled pure oxygen for 4 h.Morris water maze was performed 24 h after sevoflurane or pure oxygen inhalation.The phosphorylation of tau at Ser262 and Ser396 was detected by Western blot on 1 day after pure oxygen inhalation (T1) in groups AC and C,and on 1,3 and 7 days after sevoflurane inhalation in groups AS and S.Results Compared with group C,the escape latency was significantly prolonged and the duration of staying at the original platform quadrant was shortened in groups S and AC,and the phosphorylation of tau at Ser262 in group S and phosphorylation of tau at Ser262 and Ser396 in group AS were increased (P ＜0.05).Compared with group S,the escape latency was significantly prolonged,the duration of staying at the original platform quadrant was shortened and the phosphorylation of tau at Ser262 and Ser396 was increased in group AS (P ＜ 0.05).Compared with group AC,the escape latency was significantly prolonged,the duration of staying at the original platform quadrant was shortened and the phosphorylation of tau at Ser262 and Ser396 was increased in group AS (P ＜ 0.05).Conclusion Sevoflurane anesthesia can aggravate the impairment of cognitive function in APP positive mice and the increase in the phosphorylation of tau at Ser262 and Ser396 is involved in the mechanism.
