Effect of remifentanil on nucleotide-binding oligomerization domain 1 mRNA expression in rats with renal ischemia-reperfusion injury
10.3760/cma.j.issn.0254-1416.2012.11.031
- VernacularTitle:瑞芬太尼对肾脏缺血再灌注损伤大鼠核苷酸结合寡聚化结构域1mRNA表达的影响
- Author:
Ye MENG
;
Yanxia Lü
;
Xiaoxue JIN
;
Yingfen XIONG
- Publication Type:Journal Article
- Keywords:
Piperidines;
Nod1 signaling adaptor protein;
Reperfusion injury;
Kidney
- From:
Chinese Journal of Anesthesiology
2012;(11):1393-1396
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of remifentanil on nucleotide-binding oligomerization domain 1 (NOD1) mRNA expression in rats with renal ischemia-reperfusion (I/R) injury.Methods Sixty male Sprague-Dawley rats,weighing 220-250 g,were randomly divided into 3 groups (n =20 each):sham operation group (S group),I/R group and remifentanil group (R group).Renal ischemia was induced by occlusion of bilateral renal arteries for 45 min followed by 24 h reperfusion in groups I/R and R.Remifentanil 1.0 μg· kg-1 · min-1 was infused until 30 min of reperfusion starting from 15 min before ischemia in group R,while the equal volume of normal saline was given instead in S and I/R groups.The animals were sacrificed at 15 min before ischemia and at 3,6,24 h of reperfusion and the kidneys were removed for microscopic examination and polymorphonuclear leukocyte (PMN) count and for measurement of NOD1 mRNA expression (by RT-PCR).The apoptotic rate was determined by flow cytometry double staining method.Results Compared with group S,NOD1 mRNA expression was up-regulated,and the apoptotic rate and PMN count were significantly increased at each time point during reperfusion in group I/R,and the apoptotic rate and PMN count were significantly increased at each time point during reperfusion,and NOD1 mRNA expression was up-regulated at 6 and 24 h of reperfusion in group R (P ＜ 0.01).Compared with I/R group,NOD1 mRNA expression was down-regulated,and the apoptotic rate and PMN count were significantly decreased at each time point during reperfusion (P ＜ 0.05 or 0.01),and the pathological changes were significantly attenuated in group R.Conclusion Remifentanil can reduce the renal I/R injury by down-regulating the expression of NOD1 mRNA and inhibiting inflammatory response and apoptosis.
