Effect of inhalation of hydrogen on inflammation response during sepsis-induced acute lung injury in mice
10.3760/cma.j.issn.0254-1416.2012.11.029
- VernacularTitle:吸入氢气对脓毒症小鼠急性肺损伤时炎性反应的影响
- Author:
Hongwei LIU
;
Yuan SHI
;
Keliang XIE
;
Yonghao YU
;
Tao WANG
;
Huanzhi HAN
- Publication Type:Journal Article
- Keywords:
Hydrogen;
Sepsis;
Respiratory distress syndrome,adult;
Systemic inflammatory response syndrome
- From:
Chinese Journal of Anesthesiology
2012;(11):1387-1389
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of inhalation of hydrogen (H2) on the inflammation response during sepsis-induced acute lung injury in mice.Methods Forty-eight male ICR mice,aged 5 weeks,weighing 20-25 g,were randomly divided into 4 groups (n =12 each):sham operation group (group A),sham operation + H2 group (group B),sepsis group (group C) and sepsis + H2 group (group D).Sepsis was produced by cecal ligation and puncture (CLP).Groups B and D received 1 h inhalation of 2 ％ H2 at 1 and 6 h after CLP or sham operation.The behavior was assessed and scored at 24 h after CLP.Arterial blood samples were taken for blood gas analysis.Oxygenation index was calculated.Venous blood samples were taken for determination of the levels of serum tumor necrosis factor-α (TNF-α),interleukin-1 (IL-1β),high mobility group protein B1 (HMGB1) and interleukin-10 (IL-10).The mice were then sacrificed and lungs were removed for determination of the levels of TNF-α,IL-1β,HMGB1 and IL-10 in lung tissues.The apoptotic index was calculated.Results Compared with group A,the behavior score,apoptotic index and levels of TNF-α,IL-1β,HMGB1 and IL-10 were increased,and the oxygenation index was decreased in groups C and D,and no significant change was found in the parameters mentioned above in group B.Compare with group C,the oxygenation index and level of IL-10 were significantly increased,and the behavior score,apoptotic index and levels of TNF-α,IL-1β and HMGB1 were significantly decreased in group D.Conclusion Inhalation of H2 can regulate the balance between pro-and anti-inflammatory cytokines,inhibit the inflammation response and alleviate sepsis-induced acute lung injury in mice.
