Role of high mobility group protein box 1 in pulmonary vascular remodeling in a rat model of acute lung injury
10.3760/cma.j.issn.0254-1416.2012.10.032
- VernacularTitle:高迁移率族蛋白1在内毒素性急性肺损伤大鼠肺血管重构中的作用
- Author:
Huanliang WANG
;
Liping PENG
;
Manyi SUN
;
Wenjuan CHEN
;
Weifu LEI
;
Baozhu SUN
;
Jianbo WU
;
Wenhua ZHANG
- Publication Type:Journal Article
- Keywords:
High mobility group proteins;
Respiratory distress syndrome,adult;
Endotoxemia;
Pulmonary vascular remodeling
- From:
Chinese Journal of Anesthesiology
2012;(10):1278-1280
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of high mobility group protein box 1 (HMGB1) in pulmonary vascular remodeling in a rat model of acute lung injury (ALI).Methods Thirty healthy pathogen free male Wistar rats weighing 220-250 g were randomly divided into 3 groups (n =10 each) ∶ group control (group C) ;group LPS (group M) and group LPS + HMGB1 antibody (group H).The animals were anesthetized with intraperitoneal 10％ chloral hydrate 7 ml/kg.ALI was induced with LPS 1 mg/kg infused iv over 30 min in groups M and H.In group H HMGB1 antibody 2 mg/kg was injected iv at 12,24 and 36 h after LPS administration respectively.The animals were sacrificed at 72 h after LPS administration.The left lung was removed for microscopic examination,measurement of the thickness of the medial layer (tunica media) of pulmonary arterioles and determination of the expression of PCNA (by immune-histochemistry) and HMGB1 protein (by Western blotting).Results The medial layer of pulmonary arterioles was significantly thicker and the expression of PCNA and HMGB1 higher in group M than in group C.LPS also induced significant inflammatory cell infiltration within the alveoli and damage to the septa.In group H HMGB1 antibody significantly attenuated the above-mentioned LPS-induced changes.Conclusion HMGB1 may play an important role in the LPS-induced pulmonary vascular remodeling.
