Expression of hypoxia-inducible factor-1α within rat cerebral cortical neurons after hypoxia
10.3760/cma.j.issn.0254-9026.2012.12.025
- VernacularTitle:大鼠大脑皮层神经元缺氧后缺氧诱导因子-1α的表达
- Author:
Zhehua ZOU
;
Tao TAO
;
Jun LI
;
Jian XU
- Publication Type:Journal Article
- Keywords:
Hypoxia-inducible factor1,alpha subunit;
Neurons;
Hypoxia,brain
- From:
Chinese Journal of Geriatrics
2012;(12):1117-1119
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of hypoxia-inducible factor 1α (HIF-1α)gene in rat cerebral cortex neurons under hypoxia conditions,and provide more experimental basis for clinical treatment of hypoxic brain disease.Methods The rat cerebral cortical neurons in primary culture and hypoxia model were prepared and identified by immunocytochemistry analysis.The expression of HIF-1α in normal and hypoxic neurons was detected at 4 time points (12 h,24 h,48 h and 72 h) by immunohistochemical analysis.Results The expression of HIF-1α positive cells in normal control (NC) group was less at each time point,and it showed no statistically significant within groups.The weak expression of HIF-1α was found at 12 h in hypoxic group,and the expression augmented along with the time extended,it increased obviously at 24 h,reached the peak at 48 h(IOD＝0.27±0.02,F＝35.703,t＝11.795,P＜0.01),and declined until 72 h,it showed statistical significance between two groups at each time point.Conclusions The expression of HIF-1α is increased after hypoxia.HIF-1α has a protective effect on neurons after hypoxia.
