Hypertension activates vessel wall oxygen and metabolism sensing pathways and accelerates atherosclerosis
10.3760/cma.j.issn.0254-9026.2012.12.022
- VernacularTitle:血管内压力升高致血管壁氧张力改变与动脉粥样硬化形成机制的研究
- Author:
Weidong JANG
;
Aiqiong QIN
;
Yusheng LIU
;
Xin WANG
;
Qinghua LU
;
Zhiming GE
- Publication Type:Journal Article
- Keywords:
Hypertension;
Hypoxia-inducible factor 1,alpha subunit;
AMP-activated protein kinases;
Atherosclerosis
- From:
Chinese Journal of Geriatrics
2012;(12):1103-1107
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the molecular mechanism of atherosclerosis induced by intravascular pressure.Methods Technic aortic coarctation (TAC) was performed in ApoE-/-mice (n＝8) and control littermate (n＝8) mice.HE staining was performed in the vessels upstream and downstream of the mice models.In vitro,hypoxia inducible factor-1a (HIF-1α),heme oxygenase,reactive oxygen species and phosphorylated AMP activated protein kinase (AMPK) were analyzed in different intravascular pressure with a myograph system that allowed independent variation of flow and pressure.Results After one month of TAC,ApoE/ mice in a normal chow diet developed occlusive plaque and accelerated atherosclerotic lesions exclusively in upstream high-pressure vessel segments.In vitro,HIF-1α was increased,heme oxygenase was higher over(2.7 ±0.6) fold,reactive oxygen species and phosphorylated AMPK were also enhanced in high intravascular pressure perfused vessel segments as compared with low intravascular pressure perfused vessel segments (all P＜0.05).Conclusions Intravascular pressure elevation can activate hypoxia and metabolism-associated pathways in the arterial wall,and predispose atherosclerosis accelerated.
