Relationship between HBV polymerase gene mutations and B, C genotypes in adefovir dipivoxil-resistant chronic hepatitis B patients
10.3760/cma.j.issn.1674-2397.2012.06.005
- VernacularTitle:耐阿德福韦酯乙型肝炎病毒多聚酶区基因突变模式与病毒B、C基因型相关性分析
- Author:
Pengjian WENG
;
Guosheng GAO
;
Shixiong DING
;
Yanmin FU
;
Raodong ZHOU
;
Liangang MAO
;
Airong HU
- Publication Type:Journal Article
- Keywords:
Hepatitis B virus;
Adefovir dipivoxil;
Drug resistance;
Mutation;
Genotype
- From:
Chinese Journal of Clinical Infectious Diseases
2012;(6):342-345
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between HBV polymerase gene mutations and HBV genotypes in chronic hepatitis B (CHB) patients resistant to adefovir dipivoxil (ADV).Methods Blood samples were collected from 114 ADV-resistant CHB patients during February 2010 and May 2012.The HBV polymerase regions from serum samples were amplified with real-time PCR,and the PCR products were sequenced.Normal distribution data were presented as x ± s,and non-normal distribution data were presented as M (P25-P75) ; for homogeneous data analysis of variance and LSD-t test were performed.Results There were 8 types of HBV polymerase gene mutations in 114 CHB patients; single point mutation was detected in 102 patients (89.47％) and double or triple points mutations were detected in 12 patients (10.53％).rtA181V/T/S (57.89％),rtN236T (14.91％) and rtA181V/T/S + N236T (9.65％) were the predominant mutations.For 21 patients (18.42％) with HBV genotype B,rtN236T mutation was prevalent (47.62％,10/21) ; while for those with HBV genotype C (93,81.58％),rtA181V/T/S mutation was the predominant (65.59％,61/93).The differences of rtA181V/T/S (x2 =12.269,P ＜0.01) and rtN236T (x2 =18.658,P ＜0.01) mutation rates between B and C genotypes were statistically significant.Conclusion rtA181V/T/S,rtN236T and rtA181V/T/S + rtN236T are the major HBV polymerase gene mutation types in ADV resistant CHB patients,and mutation types are related with HBV genotypes.
