Serum metabolic profiling and screening of characteristic metabolites before and after partial hepatectomy for HBV-related liver cancer
10.3760/cma.j.issn.1007-8118.2013.02.001
- VernacularTitle:乙肝相关性肝癌手术前后血清代谢轮廓分析及特征代谢物筛选
- Author:
Li ZHANG
;
Yanan MA
;
Lei ZHANG
;
Yufan WANG
;
Jing WU
;
Ya HUANG
;
Hua KANG
;
Shuye LIU
- Publication Type:Journal Article
- Keywords:
Hepatocellular carcinoma;
Metabolomics;
Hepatitis B;
Liquid chromatogram;
Mass spectrum
- From:
Chinese Journal of Hepatobiliary Surgery
2013;(2):81-87
- CountryChina
- Language:Chinese
-
Abstract:
Objectives To investigate the characteristics of changes in serum metabolic profile before and after resection of carcinoma tissues to establish a disease distinguishing model,to analyze the changing trend of characteristic metabolites,and to determine the molecular mechanism and potential clinical value of characteristic metabolic markers for HBV-related liver cancer.Methods Ultraperformance liquid chromatography-mass spectrometry (UPLC-MS) was used to analyze the serum metabolites of 15 patients with hepatocellular carcinoma (HCC) before and after partial hepatectomy and on 25 healthy volunteers.The pattern recognition method and nonparametric test analyzes were used to analyze the data and to identify the specific metabolites and their changes after resection of carcinoma tissues.Results We established the principal component analysis (PCA) and orthogonal partial least squares-discriminant analysis (OPLS-DA) disease distinguishing model for HCC patients before and after operation as against the healthy volunteers.To distinguish between the liver cancer group and the normal control group,27 characteristic metabolites were selected from the patients before and after resection of carcinoma tissues.Eight moved towards the normal control after resection of carcinoma tissues.This indicated that liver carcinoma was an important impacting factor for these metabolites.Finally,7 metabolites were identified,and these metabolites had high diagnostic value as shown on ROC curves.Conclusions Through serum metabolic profiling of patients before and after resection of carcinoma tissues,a high correlation between metabolism and hepatocellular carcinoma was found.Researches on endogenous metabolites and pathways in liver diseases will provide a better understanding of the molecular mechanisms and provide further directions for clinical diagnosis and treatment.
