The prevalence and dynamics of human immunodeficiency virus-1 subtypes among men who have sex with men in Beijing
10.3760/cma.j.issn.1000-6680.2012.11.007
- VernacularTitle:北京市男男性行为者中人类免疫缺陷病毒1的流行亚型及其变化趋势
- Author:
Wanhai WANG
;
Liang MING
;
Shulin JIANG
;
Xiaoxi ZHANG
;
Yiming SHAO
;
Jianqing XU
;
Quancheng KAN
;
Xiaoyan ZHANG
- Publication Type:Journal Article
- Keywords:
Homosexuality,male;
HIV-1;
Genotype;
Trends
- From:
Chinese Journal of Infectious Diseases
2012;(11):673-676
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the subtype distribution and changing trend of human immunodeficiency virus (HIV)-1 strains among men who have sex with men (MSM) during 2005-2011 in Beijing.Methods Five serial cross-sectional surveys of MSM were conducted in the year of 2005-2006,2007,2008,2009,and 2010-2011 in Chaoyang district of Beijing.Whole blood samples were collected and then RNA was extracted.HIV-1 gag gene was characterized by reverse transcriptase and nested polymerase chain reaction (RT-PCR) amplification,DNA sequencing,and phylogenetic analysis of viral sequences to determine the HIV-1 subtypes.Results Phylogenetic analysis of the sequences revealed that the predominant subtypes of HIV-1 gag gene included subtype B,CRF01_AE and CRF07_BC.And CRF15_01B was detected from the year of 2008.In addition,significant changes of the distributions of subtypes and CRFs occurred from 2005 to 2011 in HIV+ MSM.Subtype B showed a significant decreased trend,while the proportions of CRF01 _AE and CRF07_BC significantly increased in the 7-year period,particularly that of CRF01_AE.Conclusions The substantial changes are observed in the diversity of HIV-1 strains circulating among MSM in Beijing during a 7-year period.
