The inhibitory effects of N-myc down-stream regulated gene-2 on proliferation of bladder carcinoma cell line T24 in vitro
10.3760/cma.j.issn.1008-1372.2012.12.001
- VernacularTitle:抑癌候选基因NDRG2在膀胱肿瘤中的表达研究
- Author:
Ruixiao LI
;
Chuigong YU
;
Jing ZHANG
;
Jianlin YUAN
;
Guojun WU
- Publication Type:Journal Article
- Keywords:
Urinary bladder neoplasms/genetics;
Urinary bladder neoplasms/metabolism;
Urinary bladder neoplasms/pathology;
Gene expression;
Immunohistochemistry;
Neoplasm proteins/biosynthesis
- From:
Journal of Chinese Physician
2012;(12):1585-1588
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the expression of a new candidate tumor suppressor N-myc downstream regulated gene 2 (NDRG2) in bladder cancer tissues and to investigate its clinical and pathological significance.Methods Formalin-fixed,paraffin-embedded tissue sections from 62 cases of bladder carcinomas and 10 cases of normal bladder tissues were analyzed retrospectively with immunohistochemistry (S-P method).Results The NDRG2 gene was highly expressed in normal bladder tissues,but low expressed in bladder carcinoma tissues.Positive expression of NDRG2 was detected in 8 of the 10 (80.0％) normal tissues and 40.3％ in bladder carcinoma ones (x2 =3.98,P ＜0.05).Furthermore,with the degree of malignancy increased,the positive expression of NDRG2 in bladder carcinoma samples was decreased.The expression of NDRG2 in bladder carcinoma was negatively correlated(r =-0.288,P ＜0.05) with C-myc(r =-0.436,P ＜0.01) and positively correlated with p53 in bladder carcinoma tissues(r =0.717,P ＜0.01).Conclusions The level of NDRG2 expression was lower in bladder carcinomas than in normal tissues.NDRG2 may play an important role in bladder carcinogenesis and in the progress of bladder cancers.
