Inhibitory effect of cisplatin combined with Astragalus on human laryngeal carcinoma Hep-2 cells induced by
10.3760/cma.j.issn.1008-1372.2012.10.016
- VernacularTitle:黄芪联合顺铂对喉癌Hep-2细胞的体外抑制作用
- Author:
Yan SONG
;
Xiuping LIU
;
Weiliang BAI
;
Wenyue JI
- Publication Type:Journal Article
- Keywords:
Laryngeal neoplasms/drug therapy;
Laryngeal neoplasms/ZHONG YAO LIAO FA;
Cisplatin/pharmacology;
Laryngeal neoplasms/pathology;
ASTRAGALUS MEMBRANACEUS △/pharmacology
- From:
Journal of Chinese Physician
2012;(10):1351-1353
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the anti-proliferation and apoptosis induction effect of cisplatin combined with Astragalus on human laryngeal cancer Hep-2 cells.Methods The proliferation inhibition of cisplatin and Astragalus alone or in combination on Laryngeal cancer Hep-2cells was measured by MTT assay.Effects of cisplatin and Astragalus alone or in combination on apoptosis of Hep-2 cells were detected by flow cytometry (FCM).Western blot was used to analyze the expressions of Bcl-2 and Bax proteins.Results The inhibition ratio of proliferation of Hep-2 cells was (53.83 ± 17.12) ％ in the astragalus group,(69.12 ± 27.12)％ in the cisplatin group,and (84.55 ± 27.84)％ in the cisplatin combined with Astragalus group,and was significantly greater than the control group (0％) (t =16.87,16.67,40.90,P ＜0.01),respectively.The apoptotic ratio of Hep-2 cells was (38.2 ± 13.6)％ in the astragalus group,(67.2 ± 17.8)％ in the cisplatin group,and (86.4 ± 25.1)％] in the cisplatin combined with Astragalus group,and was significantly greater than control group (17.1 ± 1.3) ％ (t =8.11,12.77,24.92,P ＜0.05),respectively.The effect in the combination group is better than the other group (t =11.33,9.37,P ＜ 0.01).The expressions of Bcl-2 and Bax proteins were changed.Conclusions The tumor-killing effect of cisplatin on laryngeal cancer Hep-2 cells could be enhanced significantly by the combination application of astragalus by the way of regulating the expression of Bcl-2/Bax.
