Influence of EA at different therapeutic time windows on the expression of HSP70 and nervous cell apoptosis after focal cerebral ischemic reperfusion injury in rats
10.3760/cma.j.issn.1673-4246.2012.12.011
- VernacularTitle:不同时间窗电针对脑缺血再灌注大鼠脑组织中HSP70表达及神经元凋亡的影响
- Author:
Rui CHEN
;
Jing CAI
;
Guangyi WANG
- Publication Type:Journal Article
- Keywords:
Electroacupuncure;
Focal cerebral ischemic reperfusion injury;
Different therapeutic time window;
HSP70;
Apoptosis
- From:
International Journal of Traditional Chinese Medicine
2012;(12):1091-1094
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the influence of electroacupuncure(EA) at different therapeutic time windows on the effects of inosine on neuronal apoptosis and expression of heat-shock-proteins-70 (HSP70)after focal cerebral ischemic reperfusion injury (CIRI) in rats.Methods The model was established by ligation of the artery for 2 hour.EA was delivered to “baihui” and “dazhui” through acupuncture needles 0.5 hr,2 hr,6 hr and 12 hr respectively following MCAO.The expression level of HSP70 and the number of apoptotic cells were examined by immunohistochemical technique and TUNEL,comparing the average optical density of HSP70 and the number of apoptotic positive cells of cortex penumbra and hippocampus CA1 area in rat brain.Results Compared with the model group,the average optical density of HSP70 in every EA group was increased in apoptotic positive cells of cortex penumbra and hippocampus CA1 area 0.5 h (89.98± 6.55),(128.73 ± 8.03),2 h (90.96±6.38),(132.25±8.78),6 h (93.71±6.12),(132.58±7.04),12 (96.19±7.30),(133.57±6.19)and the number of apoptotic positive cells of cortex penumbra in every EA group was decreased 0.5 h (1.80±0.84),2 h (3.40± 1.14),6 h (5.00± 1.00),12 h (5.00±2.45).The number of apoptotic cells of hippocampus CA1 area was decreased just in the EA group of 0.5 h (1.60± 1.89).Conclusion EA could increase the expression level of HSP70 and at the same time decrease the number of apoptotic cells,EA plays an important part in protecting the neuronal cells of brain after focal cerebral ischemic reperfusion injury.The acupuncture treatment should be taken as far as early.
